Clodronate is very effective in the treatment of hypercalcemia and cancer metastases to bones. It can significantly reduce the development of new metastases, as well as to decrease the risk of bone fractures, which is very high in such cases. Besides, it may also reduce the pain associated with this disease. Clodronate liposome, also known as clodrosome, seems to be the best way of delivering this compound to tissues.
To be closed in vivo, this effective compound is delivered encapsulated within liposomes. A liposome is lipid-based tiny particle, designed to carry different compounds in form of suspension. It is lately used in many research laboratories, as highly effective way or delivering drugs to various tissues. In this case, to bones and kidneys.
Although clodronate liposome, or clodrosome, achieves good results while injected, manipulation itself is associated with some risks. It may cause skin irritation, and it also may be harmful if absorbed through the skin. It irritates mucous membranes, and can be harmful if inhaled or swallowed. It can also cause eye irritations. That's why it is necessary to use respirators, chemical safety goggles, appropriate clothing and chemical-resistant gloves.
In case of oral exposure, you should wash out mouth with water and call a physician. If your eyes were in contact with this compound, you should flush them with water for at least fifteen minutes. Dermal exposure should be treated with soap and water. If inhaled, it may cause breathing problems. Exposed person should get to fresh air, and sometimes it might be necessary to use respirator or even oxygen.
Suppression of macrophage function is necessary for delivering drug to tissues. Macrophages are an essential part of natural immune system, but they also participate in or control other functions. They ingest and digest different microorganisms, and that's why you need to encapsulate some compounds within liposomes, to get through this macrophage layer.
When clodronate is encapsulated within liposomes, it can be delivered to bones and kidneys. Macrophages will digest those particles, but this compound will ve released and accumulated in tissues. Creating macrophage depleted tissues and organs this way allows more successful research of macrophage functional aspects.
Clodronate liposomes application can also be used for suppression of macrophage activity in different models of autoimmune diseases, in gene therapy and similar researches. Although this method is designed especially for in vivo research, it can also be successfully used for depletion of macrophages in vitro.
It is important to keep the suspension at appropriate temperature. It shouldn't be exposed to temperatures above 30 degrees of Celsius, or frozen. The ideal storage temperature is four degrees Celsius, and in this case it can be kept one month in refrigerator. For achieving desired results, the suspension should be kept outside the refrigerator until it reaches room temperature, before injecting it to animals.
The suspension should be well shaken before injected, in order to get homogeneous suspension of the liposomes. The skin should be shaved and treated with alcohol, to decrease the risk of infections. You should use clean syringe for injecting clodronate liposome to all animals, and follow the procedure correctly. Other ways it may come to different dosages, especially if you use the same syringe for them all.
To be closed in vivo, this effective compound is delivered encapsulated within liposomes. A liposome is lipid-based tiny particle, designed to carry different compounds in form of suspension. It is lately used in many research laboratories, as highly effective way or delivering drugs to various tissues. In this case, to bones and kidneys.
Although clodronate liposome, or clodrosome, achieves good results while injected, manipulation itself is associated with some risks. It may cause skin irritation, and it also may be harmful if absorbed through the skin. It irritates mucous membranes, and can be harmful if inhaled or swallowed. It can also cause eye irritations. That's why it is necessary to use respirators, chemical safety goggles, appropriate clothing and chemical-resistant gloves.
In case of oral exposure, you should wash out mouth with water and call a physician. If your eyes were in contact with this compound, you should flush them with water for at least fifteen minutes. Dermal exposure should be treated with soap and water. If inhaled, it may cause breathing problems. Exposed person should get to fresh air, and sometimes it might be necessary to use respirator or even oxygen.
Suppression of macrophage function is necessary for delivering drug to tissues. Macrophages are an essential part of natural immune system, but they also participate in or control other functions. They ingest and digest different microorganisms, and that's why you need to encapsulate some compounds within liposomes, to get through this macrophage layer.
When clodronate is encapsulated within liposomes, it can be delivered to bones and kidneys. Macrophages will digest those particles, but this compound will ve released and accumulated in tissues. Creating macrophage depleted tissues and organs this way allows more successful research of macrophage functional aspects.
Clodronate liposomes application can also be used for suppression of macrophage activity in different models of autoimmune diseases, in gene therapy and similar researches. Although this method is designed especially for in vivo research, it can also be successfully used for depletion of macrophages in vitro.
It is important to keep the suspension at appropriate temperature. It shouldn't be exposed to temperatures above 30 degrees of Celsius, or frozen. The ideal storage temperature is four degrees Celsius, and in this case it can be kept one month in refrigerator. For achieving desired results, the suspension should be kept outside the refrigerator until it reaches room temperature, before injecting it to animals.
The suspension should be well shaken before injected, in order to get homogeneous suspension of the liposomes. The skin should be shaved and treated with alcohol, to decrease the risk of infections. You should use clean syringe for injecting clodronate liposome to all animals, and follow the procedure correctly. Other ways it may come to different dosages, especially if you use the same syringe for them all.
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